One of my favorite movie vampire bad asses has to be Wesley Snipes as Blade (1998). The opening scene at the vampire disco is classic and vamp hottie Stephen Dorff is amazing in this as well. I also loved the characters played by Kris Kristofferson and Donal Logue. Great plot, great action, just a great flick! I saw Blade II (2002) but don't remember much about it anymore. I do remember Blade Trinity (2004). How could you forget Parker Posey as a nasty vampire chick or Ryan Reynolds as a vampire hunter? Awesome sauce!
They don't make vampires like Blade anymore, I mean, check out those sunglasses! *Sigh* Maybe they could put out just one more film...(hint hint, if you're listening, Hollywood!)